- ￥332
- hyclone
- 美国
- SH30824.01
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
充足
- 供应商：
hyclone
- CAS号：
SH30824.01
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文献和实验-well microplates at a density of 104 per well and were cultured in cell culture medium for 10–12 h. After treatment with clivorine for 24, 48 and 72 h,500 mg/ml 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) was added and was incubated
a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
Sample preparation (analytical gels)
(cerebrospinal fluid): An aliquot of 250 μl of human CSF was mixed with 500 μl of ice cold acetone and centrifuged at 10000 g at 4o C for 10 minutes. The pellet was mixed with 10 μl of a solution containing SDS (10% w/v) and DTE (2.3% w/v). The sample was heated
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