- ¥5627
- OXOID
- CM1053R
- 2025年11月25日
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- 英文名：
DEMI-FRASER BROTH(W/O FERRIC AMMON CITR)
- 供应商：
OXOID
英文名称：DEMI-FRASER BROTH(W/O FERRIC AMMON CITR)
中文名称：DEMI-FRASER BROTH(W/O FERRIC AMMON CITR)
生产商： OXOID
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价 格：¥5627
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文献和实验.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical
Protein Expression and Purification Protocol
. (Have a stock of ampicillin in the 4o C which is 1000x concentrated or 100 mg/ml in sterile distilled water to make things easier). b) Pick a colony or two from your plate using a sterile innoculation loop and add to the LB broth/ampicillin flask. Incubate
How to Make Competent CellsStep 1: Take an 100 m L aliquot of frozen cells from the -80o C and innoculate about 500 ml to 1 L sterile LB broth . DO NOT ADD antibiotic, since these cells do not have an plasmid in them. Work as sterile as possible
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