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Apogee超灵敏纳米流式细胞仪
市场唯一能检测小至70nm小颗粒的流式细胞仪
微生物、细胞外囊泡、外泌体、大病毒、纳米颗粒、动物细胞、人类细胞等
市面上大部分流式细胞仪主要针对细胞而设计,散射光的检测极限通常是500nm附近,检测500nm以下的生物颗粒通常依靠荧光探针,可是有时不能完全依靠荧光标记来区分不同细胞。这是多方面的原因决定的,如非特异性结合,细胞碎片的存在,结块的抗体或者荧光染料的摄取低下等,因此只有结合散射光才能得到理想的结果。;然而很多生物颗粒如细胞外囊泡,直径一般在300nm以下,比细胞小得多,产生的散射光信号非常微弱,故难以被传统流式细胞仪检测到。Apogee超灵敏纳米流式细胞仪具有非常好的散射光和荧光灵敏度和分辨率,可检测从细胞到小至70nm的小颗粒,为亚微米生物颗(100nm-1000nm)粒检测带来了新希望!
80nm灵敏度/10nm分辨率 Apogee超灵敏纳米流式细胞仪在亚微米生物颗粒检测领域中独领风骚
·第一个拥有80nm散射光灵敏度
·非常适合微生物,细胞外囊泡,纳米颗粒&大型病毒检测
·同样适用于常规流式应用,不仅限于微小颗粒
细胞微粒(Microparticles)和外泌体(Exosomes)
·2个或3个光散射角度范围带来极高的灵敏度
·微泡可用于第二代诊断
·基于血液的癌症诊断检测
·血管生物学和血栓形成研究
·治疗药物给药
血小板
A50-Micro的高灵敏度非常适合血小板应用:
·血小板反应性
·血小板聚集
·循环活化血小板
·血小板微颗粒
·钙流
·细菌污染
微生物/病毒(如:巨细胞病毒)
·各种样品中细菌的快速检测
·细胞周期分析和遗传学
·细胞活性
·荧光蛋白
·病毒学
动物/人类细胞
常规流式分析:细胞凋亡/活力、线粒体膜电位分析、植物倍体分析、DNA含量分析等
技术参数
灵敏度:散射光灵敏度:Micro Plus :70nm乳胶微球
Micro :110nm乳胶微球
荧光灵敏度:100 MESF
分辨率:10nm乳胶微球(用散射光)
配置:多达9个荧光检测器
多达3个散射光检测器
多达3个激光器
可选配自动进样器(兼容96孔板上样)
体积化进样系统,对所有感兴趣的群体都可绝对计数
速度:>100,000事件/秒
多达45个样品/小时
可调节进样量:50-400ul(可选配至900ul)
软件控制进样速度:0.7-150ul/min
允许样品浓度上限:109/ml
鞘液:封闭循环超纯鞘液系统,可填充鞘液桶
废液:无特殊要求
如需要可在废液桶中加入漂白剂
废液桶高度不影响仪器性能
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