Apogee超灵敏纳米流式细胞仪
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Apogee超灵敏纳米流式细胞仪

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    Apogee超灵敏纳米流式细胞仪

    市场唯一能检测小至70nm小颗粒的流式细胞仪
    微生物、细胞外囊泡、外泌体、大病毒、纳米颗粒、动物细胞、人类细胞等

    市面上大部分流式细胞仪主要针对细胞而设计,散射光的检测极限通常是500nm附近,检测500nm以下的生物颗粒通常依靠荧光探针,可是有时不能完全依靠荧光标记来区分不同细胞。这是多方面的原因决定的,如非特异性结合,细胞碎片的存在,结块的抗体或者荧光染料的摄取低下等,因此只有结合散射光才能得到理想的结果。;然而很多生物颗粒如细胞外囊泡,直径一般在300nm以下,比细胞小得多,产生的散射光信号非常微弱,故难以被传统流式细胞仪检测到。Apogee超灵敏纳米流式细胞仪具有非常好的散射光和荧光灵敏度和分辨率,可检测从细胞到小至70nm的小颗粒,为亚微米生物颗(100nm-1000nm)粒检测带来了新希望!

    80nm灵敏度/10nm分辨率 Apogee超灵敏纳米流式细胞仪在亚微米生物颗粒检测领域中独领风骚

    ·第一个拥有80nm散射光灵敏度
    ·非常适合微生物,细胞外囊泡,纳米颗粒&大型病毒检测
    ·同样适用于常规流式应用,不仅限于微小颗粒

    细胞微粒(Microparticles)和外泌体(Exosomes)
    ·2个或3个光散射角度范围带来极高的灵敏度
    ·微泡可用于第二代诊断
    ·基于血液的癌症诊断检测
    ·血管生物学和血栓形成研究
    ·治疗药物给药

    血小板
    A50-Micro的高灵敏度非常适合血小板应用:
    ·血小板反应性
    ·血小板聚集
    ·循环活化血小板
    ·血小板微颗粒
    ·钙流
    ·细菌污染

    微生物/病毒(如:巨细胞病毒)
    ·各种样品中细菌的快速检测
    ·细胞周期分析和遗传学
    ·细胞活性
    ·荧光蛋白
    ·病毒学


    动物/人类细胞
    常规流式分析:细胞凋亡/活力、线粒体膜电位分析、植物倍体分析、DNA含量分析等

    技术参数

    灵敏度:散射光灵敏度:Micro Plus :70nm乳胶微球
    Micro :110nm乳胶微球
    荧光灵敏度:100 MESF
    分辨率:10nm乳胶微球(用散射光)
    配置:多达9个荧光检测器
    多达3个散射光检测器
    多达3个激光器
    可选配自动进样器(兼容96孔板上样)
    体积化进样系统,对所有感兴趣的群体都可绝对计数
    速度:>100,000事件/秒
    多达45个样品/小时
    可调节进样量:50-400ul(可选配至900ul)
    软件控制进样速度:0.7-150ul/min
    允许样品浓度上限:109/ml
    鞘液:封闭循环超纯鞘液系统,可填充鞘液桶
    废液:无特殊要求
    如需要可在废液桶中加入漂白剂
    废液桶高度不影响仪器性能

    细胞微粒和外泌体领域:
    科家们开始发现由细胞分泌出来的微粒和外泌体在很多疾病(如心血管病、癌症、等)中有着重要的关系,所以近年微粒和外泌体的研究愈来愈火热。细胞微粒的大小一般在100nm-1000nm,而外泌体更在300nm以下,一般的流式检测在500nm大小以下的颗粒就会存在着限制。Apogee超灵敏纳米流式细胞仪系统独有的散射光技术和多角度检测器可以突破以往流式检测的限制,所有小颗粒都可无所遁形!

    Apogee超灵敏纳米流式细胞仪

    ApogeeMix珠子检测结果。珠子尺寸:110nm,180nm,240nm,300nm,500nm,590nm,880nm&1300nm并包含一种折射率与生物粒子相似的二氧化硅玻璃珠。

    微生物领域:
    Apogee超灵敏纳米流式细胞仪的光散射表现—能监测出最小的颗粒,优于市场上任何流式细胞仪,并具有极好的光散射分辨率。仅用散射光(light scatter),超灵敏纳米流式细胞仪就能对不同的细菌 (如Latobacillus 和Staphylococcus) 进行出色的分辨和绝对计数。而这在传统流式细胞仪上只能检测出一个类群(重叠),超灵敏纳米流式细胞仪能完全将两个种细胞通过两个光散射信号区分开。

    Apogee超灵敏纳米流式细胞仪

    仅用散射光,便能将乳酸菌、葡萄球菌完全区分开来

    超灵敏纳米流式细胞仪可以清晰地将直径相差10nm以上的微生物混合物进行分群、计数、分析,在微生物学、食品监测、水质监测等领域应用广泛。

    多通道荧光检测:

    Apogee超灵敏纳米流式细胞仪

    Apogee超灵敏纳米流式细胞仪可最多配备3个激光,並可同时检测9种荧光,而且有着很好的荧光灵敏度。用戶可按实验需要选择激光数量和波长,常用激光波长包括:375nm、405nm、488nm、532nm、552nm、635nm等。Apogee也可为用戶的要求去提供需要的波长范围的激光。

    免疫细胞监控:
    CD4+T淋巴细胞是HIV感染的主要靶细胞,当人体感染了HIV后,主要表现为CD4+T淋巴细胞的丢失,绝对数量的减少,所以CD4+,CD4+T淋巴细胞计数作为直接测定免疫功能的方法,是提供HIV感染病人免疫系统损害状况最明确的指标。

    Apogee超灵敏纳米流式细胞仪

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      Apogee超灵敏纳米流式细胞仪
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