The antibody is a rabbit polyclonal antibody raised against PPARgC1a. It has been selected for its ability to recognize PPARgC1a in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体QUALITY CONTROL
Content: The quality control contains recombinant PPARgC1a (Ser573~Asn767) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体STORAGE
Store at 4oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.