大鼠肺泡Ⅱ型细胞,RLE-6TN细胞

大鼠肺泡Ⅱ型细胞,RLE-6TN细胞基本信息
资源编号 hzCC337708
资源名称 RLE-6TN
种属 大鼠肺泡Ⅱ型细胞
类型 肺泡Ⅱ型，SV40转化
形态 上皮样
提供形式 P4-5代T25细胞培养瓶
安全等级 2
模式菌株 未知
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大鼠肺泡Ⅱ型细胞,RLE-6TN细胞培养方法
培养基 90%RPMI-1640+10%FBS
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
大鼠肺泡Ⅱ型细胞,RLE-6TN细胞详细说明
Age 56 day old
Gender male
Strain Fischer 344 (F344)
Karyotype Cells are reported to remain near diploid and karyotypically stable from passage 19-70 with 50% or more of the cells containing 42 chromosomes. At passage 37, there was a translocation between chromosome 1 and 15 which results in trisomy of the q arm of chromosome 1.
Derivation Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Clinical Data male
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Genes Expressed cytokeratin 8 and 19,However, expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.,The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.,Expression of several chemotactic cytokines by RLE-6TN cells was
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
Cellular Products cytokeratin 8 and 19
Tumorigenic No
Effects No, not tumorigenic in nude mice
Passage History At passage 5, the alveolar Type II cells were transfected with SV40 (pRSV-T DNA) by lipofection.
描述 The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.;At passage 5, the alveolar Type II cells were transfected with SV40 (pRSV-T DNA) by lipofection.
The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.
Expression of several chemotactic cytokines by RLE-6TN cells was reported to be similar to that of primary cultures of alveolar Type II cells.
Although these cells appear negative for the SV40 antigen, they should be handled as potentially biohazardous material under at least Biosafety Level 2 containment.大鼠肺泡Ⅱ型细胞,RLE-6TN细胞SH-SY5Y 神经母细胞瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-231 乳腺导管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF126 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-453 乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF17 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
T47D 乳腺导管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF763 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
ZR-75-1 乳腺导管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF767 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T3 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L929 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T3/e 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Mo-MuLV/3T3 Mo-MuLV感染的3T3 品牌ATCC 规格25毫升/株. 货期1-2周
3T3TK 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
NIH3T3 NIH SWISS 小鼠胚细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T6 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PA317 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Ana-1 小鼠巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SRSV/3T3 SRSV转化的3T3 品牌ATCC 规格25毫升/株. 货期1-2周
CTLL-2 小鼠T细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHL 中国仓鼠肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BRL 肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周
NRK 鼠肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L-6TG 肌母细胞 品牌ATCC 规格25毫升/株. 货期1-2周
V79 中国仓鼠肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BHK 幼仓鼠肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHO 中国仓鼠卵巢细胞 品牌ATCC 规格25毫升/株. 货期1-2周
R1610 中国仓鼠体细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHO/dhFr- 二氢叶酸还原酶缺陷型CHO细胞 品牌ATCC 规格25毫升/株. 货期1-2周
2BS 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HLF 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
WI-38 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SL-7 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
XJH B淋巴细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L-02 肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Chang Liver 张氏肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周
QSG-7701 小肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周
H.A. 羊膜细胞 品牌ATCC 规格25毫升/株. 货期1-2周
293 Ad5DNA转化的胚肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周