RLE-6TN大鼠肺泡Ⅱ型细胞

RLE-6TN大鼠肺泡Ⅱ型细胞基本信息
资源编号 YBCC337708
资源名称 RLE-6TN
种属 大鼠肺泡Ⅱ型细胞
类型 肺泡Ⅱ型，SV40转化
形态 上皮样
提供形式 P4-5代T25细胞培养瓶
安全等级 2
RLE-6TN大鼠肺泡Ⅱ型细胞模式菌株 未知
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培养方法
培养基 90%RPMI-1640+10%FBS
生长条件 95%空气+5%二氧化碳 37摄氏度
RLE-6TN大鼠肺泡Ⅱ型细胞生长特性 贴壁
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
详细说明
Age 56 day old
Gender male
Strain Fischer 344 (F344)
Karyotype Cells are reported to remain near diploid and karyotypically stable from passage 19-70 with 50% or more of the cells containing 42 chromosomes. At passage 37, there was a translocation between chromosome 1 and 15 which results in trisomy of the q arm of chromosome 1.
Derivation Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Clinical Data male
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Genes Expressed cytokeratin 8 and 19,However, expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.,The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.,Expression of several chemotactic cytokines by RLE-6TN cells was
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
Cellular Products cytokeratin 8 and 19
Tumorigenic No
Effects No, not tumorigenic in nude mice
Passage History At passage 5, the alveolar Type II cells were transfected with SV40 (pRSV-T DNA) by lipofection.
RLE-6TN大鼠肺泡Ⅱ型细胞描述 The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.
The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization.;At passage 5, the alveolar Type II cells were transfected with SV40 (pRSV-T DNA) by lipofection.
The cell line exhibits characteristics of alveolar Type II cells such as lipid-containing inclusion bodies (phosphine 3R staining and electron microscopy) and expression of cytokeratin 8 and 19; the cells do not express alkaline phosphatase activity.
Expression of several chemotactic cytokines by RLE-6TN cells was reported to be similar to that of primary cultures of alveolar Type II cells.
Although these cells appear negative for the SV40 antigen, they should be handled as potentially biohazardous material under at least Biosafety Level 2 containment.
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