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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.
- 克隆性:
Polyclonal
- 宿主:
Chicken
- 应用范围:
User optimized
- 抗体英文名:
HA antibody (HRP)
- 抗体名:
Polyclonal HA antibody, Anti-HA antibody, HA tag antibody, HA epitope antibody, Hemagglutinin antibody
- 规格:
1 ml
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Protocol for anti-HA antibody Western Blotting
) 5) Incubate in primary antibody solution: I use PBS with 0.1% TWEEN-20 plus 5% BSA as the basic solution, but you can use TBS too. I dilute the HA antibody 1:5000, just to be sure you will see a signal. I haven't EVER seen appreciable background. I've
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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