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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
Use of E. coli for the Production of a Single Protein
only the target protein. In the SPP system, all E. coli cellular mRNAs are eliminated by the induction of MazF, an ACA-specific mRNA interferase, which results in complete cell growth arrest. However, the mRNA for a target protein was engineered to be devoid
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
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