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- Enzo
- 见包装说明
- ENZ-51031-K200
- 美国
- 2026年03月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
少量现货
- 供应商：
欣博盛生物
- 检测范围：
见包装说明
- 应用：
Flow Cytometry| Fluorescence microscopy| Fluorescent detection| HTS
- 样本：
/
- 规格：
200 tests
CYTO-ID® Autophagy detection kit，产品货号是：ENZ-51031-K200。该产品由Enzo Life Sciences生产。欣博盛生物是Enzo Life Sciences授权一级代理商，负责产品在国内的销售以及技术支持服务。如需购买该产品，或该品牌其他产品，请联系我们欣博盛生物！
A no-transfection, quantitative assay for monitoring autophagy in live cells
Rapid, no transfection required
Protocol validated with known inhibitors and activators of autophagic activity
Selective and comprehensive staining allows differentiation between autophagic flux and autophagolysosome accumulation
Negligible staining of lysosomes reduces background seen with other dyes
Facilitates high-throughput screening of activators and inhibitors of autophagy
Enzo Life Sciences CYTO-ID® Autophagy Detection Kit measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles. The 488nm-excitable green dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes). The kit also includes the Hoechst 33342 dye for the nuclear staining, an Autophagy Inducer (Rapamycin) and a Lysosomal Inhibitor (Chloroquine).
Mechanism of Action
The probe is a cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. Careful selection of titratable functional moieties on the dye prevents its accumulation within lysosomes, but enables labeling of vacuoles associated with the autophagy pathway.
Autophagy is a stress-induced protective mechanism. Less active under basal conditions, the mechanism is utilized by eukaryotic cells through lysosome-mediated bulk degradation of cellular contents when subjected to certain hostile conditions such as nutrient depletion and chemical or environmental stress. The role of increased autophagic activity in the pathology of cancer, neurodegeneration, cardiovascular disease and diabetes has become widely recognized and commonly studied. Induction of autophagic flux can be visualized by enhanced accumulation of autophagic vesicles if lysosomal function is inhibited, preventing removal of these vesicles.
Product Details
Application
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
Application Notes
The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.
Contents
CYTO-ID® Green Detection Reagent
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer
Quality Control
A sample from each lot of CYTO-ID® Autophagy detection kit is used to stain HeLa Cells as described in user manual. CYTO-ID® autophagy detection reagent is incorporated into induced cells, observed as accumulative typical spherical vacuoles in foci or throughout cytoplasm. Comparing to untreated HeLa cells, treated sample demonstrate significant increase in fluorescence under microscope.
Quantity
For -K200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.
For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays.
Technical Info / Product Notes
The CYTO-ID® Autophagy Detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.
Featured in:
Genetic Engineering & Biotechnology News – HTS Profiling Method for Autophagy-Modulators
Application Notes:
Autophagy Analysis Using Object Spot Counting Using Gen5 to Analyze the Size and Number of Autophagosomes Per Nuclei
Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation
Response Profiles of Known Autophagy-Modulators Across Multiple Cell Lines: Using CYTO-ID® Autophagy Dye to assess Compound Activity and Toxicity
Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways
A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells
Predictive High-Content/High-Throughput Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell (iPSC)-Derived Hepatocytes
Visualizing subcellular vesicles to quantitate autophagy in neuronal cells
Cited samples:
For an overview on cited samples please click here.
Handling & Storage
Use/Stability
With proper storage, the kit components are stable for one year from date of receipt.
Handling
Protect from light. Avoid freeze/thaw cycles.
Short Term Storage
-20°C
Long Term Storage
-80°C
Shipping
Blue Ice
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文献和实验- Neuroglobin regulates autophagy through mTORC1/RAPTOR/ULK-1 pathway in human neuroblastoma cells: Manganelli, V., Costanzo, M., et al.; Sci. Rep. 15, 7642 (2025), Abstract
- A sesquiterpene lactone, tomentosin, as a novel anticancer agent: orchestrating apoptosis, autophagy, and ER stress in colorectal cancer: S. Çetinkaya, et al.; Naunyn Schmiedebergs Arch Pharmacol , (2025), Abstract
- Ancient genomic linkage of α-globin and Nprl3 couples metabolism with erythropoiesis: A.E. Preston, et al.; Nat Commun 16, 2749 (2025), Abstract
- Bioengineering the metabolic network of CAR T cells with GLP-1 and Urolithin A increases persistence and long-term anti-tumor activity: A. Akhtar, et al.; Cell Rep Med 6, 102021 (2025), Abstract
- Hypoxia‑induced SREBP1‑mediated lipogenesis and autophagy promote cell survival via fatty acid oxidation in breast cancer cells: J.H. Jung, et al.; Oncol Lett 29, 175 (2025), Abstract
- Immunomodulatory Nanoparticles Induce Autophagy in Macrophages and Reduce Mycobacterium tuberculosis Burden in the Lungs of Mice: R.B. Bekale, et al.; ACS Infect Dis 11, 610 (2025), Abstract
- Mitochondrial Mayhem: How cigarette smoke induces placental dysfunction through MMS19 degradation: P. Zhou, et al.; Ecotoxicol Environ Saf 290, 117728 (2025), Abstract
- Sauchinone Ameliorates Senescence Through Reducing Mitochondrial ROS Production: M.U. Kuk, et al.; Antioxidants 14, 259 (2025), Abstract
- Waterborne ammonia toxicity damages crustacean hemocytes via lysosome-dependent autophagy: A case study of swimming crabs Portunus trituberculatus: Y. Lu, et al.; Environ Res 272, 120985 (2025), Abstract
- Enhanced cytotoxicity of T-DM1 in HER2-low carcinomas via autophagy inhibition: Zhang, J., Chang, X., et al.; PLoS One 20, e0322029 (2025), Abstract
Detection of Cell Death by Autophagy
Autophagy (Greek: Self digestion) is an intracellular process involved in removal of damaged or misfolded proteins or organelles. Damaged or misfolded proteins or organelles are first engulfed in a membraneous structure called autophagosome
The Emerging Links Between Sirtuins and Autophagy
deacetylase activity, in vivo acetylation detection, and the common assays used to monitor both autophagy and the more selective process of mitophagy.
Measurement of Autophagy in Cells and Tissues
Two major proteolysis systems, the ubiquitin-proteasome system, and the autophagy-lysosome system, contribute to degradation of various types of protein and/or protein aggregates. In general, the autophagy-lysosome system is involved in bulk
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