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文献和实验Isolation of Total RNA from Difficult Tissues
The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA and nucleases present distinct
. It is therefore prudent to investigate expression at the RNA and protein levels. This chapter will discuss the isolation of total RNA from yeast. The reader should refer to ref. (1 ) for a detailed description of the methods for preparation of ribosomal and small RNAs
DNase I Treatment Of Total RNA
（1 U/ul）1 ul 10X DNaseI bufferDEPC water to 10 ulExample of a scaled up version for a 40 ul reaction100 ug Total RNA4 ul DNase I （1 U/ul）4 ul 10X DNaseI bufferDEPC water to 40 ulPrepare your reaction and mix well.Incubate 37°C for 30 minutes. Stop
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