AMPINEXT™ High-Sensitivity Bis

Native Chromatin Preparation and Illumina/Solexa Library Construction

, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using

Evaluation of MeDIP-Chip in the Context of Whole-Genome Bisulfite Sequencing (WGBS-Seq) in Arabidopsis

(DMRs) at the genome-wide scale, and to relate this variation to gene expression and phenotypic diversity. Whole-genome bisulfite sequencing (WGBS-seq) has established itself as a gold standard in DNA methylation analysis due to its high

Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform

expression studies to personalized clinical diagnostics. Here, we describe the preparation of cDNA libraries, from both whole aphid insects and their microscopic salivary gland tissues, suitable for high-throughput DNA sequencing on the Illumina platform.