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欣博盛
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ea
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文献和实验Using RNA Interference in Schistosoma mansoni
we describe methods for applying RNAi to suppress gene expression in the intra-mammalian life stages of Schistosoma mansoni . These methods include isolating and culturing the parasites, preparing and delivering dsRNA targeting a specific gene and monitoring
The Culture of Schistosoma mansoni and Production of Life Cycle Stages
Schistosoma mansoni is a trematode parasite with a freshwater snail intermediate host but whose definitive host is human. Schistosomiasis is a chronically debilitating, and often fatal disease affecting 200- 300 million people
Generation of Parasite Antigens for Use in Toll-Like Receptor Research
Schistosoma mansoni . We also describe a protocol for generation of S. mansoni soluble egg antigens (SEA), which are commonly used in TLR research. This protocol has application for the isolation of extract from other parasites or pathogens as it is intended
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