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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
欣博盛生物
- 应用：
Colorimetric detection
- 规格：
10 assays
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
96-well RNA In Situ Hybridization Protocol
. Wash 5 min at RT with AP buffer. 24. Add 200ul AP buffer containing Nitro Blue Tetrazolium (NBT) and Bromo-Chloro- Indolyl-Phosphatase (BCIP). 25. Incubate with rocking until desired color development is achieved (20 min. to overnight
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
: 2500. Staining Solution : 100 mM NaCl; 5 mM MgCl2; 100 mM Tris (pH 9.5); 0.1% Tween 20; 1mM Levamisole. Levamisole is a potential inhibitor of endogenous phosphatases. Sigma Fast BCIP/NBT
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