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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 英文名:
Protein Ladder One, Multi-color(Broad Range) for SDS-PAGE
- 库存:
99
- 供应商:
北京绿百草科技发展有限公司
- 规格:
500UL
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文献和实验, and antibody is allowed to bind to the protein. The bound antibody is detected by the addition of a second antibody conjugated to an enzyme and an enzyme substrate with color detector is added. The relative intensity of the color developed is proportional
Co-Expression of human DNA primase p49-p58 subunits
fractions in 1.5 ml epp tubes.The majority of the protein should elute in the first ~3 ml.After elution,beads should be a light blue color. 13.Run SDS-PAGE on fractions to make sure you got everything off of column and to check purity. 14.Pool fractions
to determine sites of contact among subunits of a multi-subunit complex. By an “ordered fragment ladder” far-Western analysis, they were able to identify the interaction domains of E. coli RNA polymerase ß subunit. The protein was expressed as a polyhistidine
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