小鼠肾癌细胞,Renca细胞

小鼠肾癌细胞,Renca细胞基本信息
资源编号 hzCC337867
资源名称 Renca
种属 小鼠肾癌细胞
类型 epithelial
形态 epithelial-like
提供形式 P1-2代冻存管
致病类型 renal adenocarcinoma
安全等级 2
模式菌株 未知
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小鼠肾癌细胞,Renca细胞培养方法
培养基 90%高糖DMEM+10%FBS
传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 104 to 4 X 104 viable cells/cm2 is recommended. Incubate cultures at 37°C. We recommend that you subculture when the culture reaches a cell concentration between 8 X 104 and 1.5 X 105 cells/cm2. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁生长
存储条件 50%高糖DMEM+40%FBS+10%DMSO 液氮
小鼠肾癌细胞,Renca细胞详细说明
Age 6 weeks
Gender male
Derivation The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice.
Clinical Data male
Tumorigenic YES
Population Doubling Time approximately 24 hours
Year of Origin 1969
描述 The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver.聽RefMurphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766聽RefSalup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425聽The cells do not express transforming growth factor-beta type II receptor (TbetaR-II) 10414746.聽RefEngel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746小鼠肾癌细胞,Renca细胞HZ-pc-h028 肺泡上皮细胞 Alveolar epithelial cells
HZC3793 肺泡上皮细胞培养基 AEpiCM
HZC4122 肺泡上皮细胞培养基 AEpiCM
HZ-pc-h022 肺微血管内皮细胞 Pulmonarymicrovascularendothelialcells
HZ-pc-r001 肺微血管内皮细胞 Pulmonary microvascularendothelial cells
HZC3511 肺细胞系统
HZ-pc-h032 肺纤维原细胞 Pulmonary fibroblastscells
HZ-pc-r002 肺血管平滑肌细胞 Pulmonary vascular smooth musclecells
HZC3036 负鼠肾细胞 OK
HZC4196 甘油检测试剂盒 GLY
HZC4173 甘油醛3磷酸脱氢酶分析试剂盒 GPDH
HZC4197 "甘油三酸酯检测试剂盒
" TG
HZ-pc-h064 肝动脉内皮细胞 Hepatic arterial endothelialcells
HZ-pc-r033 肝动脉内皮细胞 Hepatic artery endothelial cells
HZ-pc-h065 肝动脉平滑肌细胞 Hepatic arterial smooth musclecells
HZ-pc-r034 肝动脉平滑肌细胞 Hepatic artery smooth musclecells
HZ-pc-h067 肝窦内皮细胞 Sinusoidal endothelial cells
HZ-pc-r037 肝窦内皮细胞 Sinusoidal endothelial cells
HZC3304 肝卵圆细胞 HOC
HZ-pc-h062 肝内胆管上皮细胞 Intrahepatic biliary epithelialcells
HZ-pc-r035 肝内胆管上皮细胞 Intrahepatic biliary epithelialcells
HZ-pc-h063 肝实质细胞 Hepatocytes cells
HZ-pc-r032 肝实质细胞 The liver parenchyma cells
HZC3804 肝细胞培养基 HM
HZC3575 肝细胞系统
HZ-pc-r038 肝星形细胞 Hepatic stellate cells
HZC4082 干细胞超低结合培养板 ULBC plates
HZC3603 干细胞衍生产品
HZC3798 睾丸间质细胞培养基 LCM
HZC3989 睾丸间质细胞生长因子 LCGS
HZ-pc-h095 睾丸支持细胞 Sertoli cells
HZC3770 狗间充质干细胞--脂肪 DMSC-ad
HZC3040 狗肾恶性组织细胞增生症细胞 DH82
HZC3315 狗肾上皮细胞 MDCK[NBL-2]
HZC3367 狗肾上皮细胞 MDCK-EGFP-puro（慢病毒构建稳定株)
HZC3037 狗肾细胞 MDCK(NBL-2)
HZC3038 狗肾细胞 Super Tube
HZC4190 谷氨酸检测试剂盒 GLU
HZC4168 谷氨酸脱氢酶检测试剂盒 GDH
HZC4200 谷胱甘肽/氧化型谷胱甘肽比率检测试剂盒 GSH/GSSG