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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
GFP 多克隆抗体(兔源)
- 抗体英文名:
GFP Antibody, pAb, Rabbit
- 靶点:
GFP
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
Western Blot、免疫细胞化学 / 免疫荧光 (ICC/IF)、免疫组化 (IHC)、免疫沉淀 (IP)、ELISA
- 宿主:
兔(Rabbit)
- 适应物种:
通用
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
人 GFP 蛋白来源合成肽
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
无(Unconjugated)
- 克隆性:
多克隆(Polyclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Rabbit IgG
- 免疫原:
人 GFP 蛋白来源合成肽
- 规格:
40μg
| Specificity | We Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv. |
| Host Species | Rabbit |
| Immunogen | Purified recombinant full-length GFP protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Affinity chromatography |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| Clone ID | Not applicable |
| Target Background | Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria . It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.We Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
| Synonyms | Rabbit Anti-GFP pAb; |
GFP 抗体、绿色荧光蛋白抗体、兔源 GFP 抗体、多克隆 GFP 抗体、抗 GFP 抗体、标签抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
GFP抗体|GFP抗体检测GFP、EGFP、YFP、EYFP、CFP抗体
EarthOx GFP标签抗体(#E022030)用于WB实验,1道为阴性对照,2道为1:1000倍稀释 我们先看一下EarthOx的单克隆GFP抗体 ――Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) (Abcam, Cambridge, UK; Sigma, St. Louis, USA.; Roche, Mannheim, Germany). We recently generated a GFP-binding protein (GBP) based on a single domain antibody derived from Lama alpaca (Rothbauer et al., 2008). This GFP-binding protein is characterized
技术资料




