- 询价
- PEPROTECH
- 中国/美国
- xy-E627Mu01
- 2026年01月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Recombinant Cyclin Dependent Kinase Inhibitor 2B (CDKN2B)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | xy-E627Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 17.5kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Met1~Asp130 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
MLGGSSDAGL ATAAARGQVE TVRQLLEAGA DPNALNRFGR RPIQVMMMGS AQVAELLLLH GAEPNCADPA TLTRPVHDAA REGFLDTLVV LHRAGARLDV CDAWGRLPVD LAEEQGHRDI ARYLHAATGD
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验、Glu、Ser和Thr的区域，且在该区域附近有某些特定的氨基酸，则该蛋白就会不稳定。这些PEST结构域的磷酸化导致钙的结合能力提高，从而利于钙依赖性蛋白酶对蛋白质的降解。这提示可以在缺乏PEST蛋白裂解系统的E.coli中表达PEST富含蛋白。 减少E.coli中重组蛋白裂解的策略有以下几种：（1）将蛋白质靶向细胞周质或培养基[145，186]；（2）在较低的温度下培养细菌[187]；（3）选用蛋白酶缺陷的菌株[188]；（4）构建N-末端或C-末端融合蛋白[186]；（5）将目的基因多拷贝串联
质不稳定性的另一个关系体现在PEST假说中[185]。根据对短寿命真核蛋白的统计分析，蛋白质如果富含Pro、Glu、Ser和Thr的区域，且在该区域附近有某些特定的氨基酸，则该蛋白就会不稳定。这些PEST结构域的磷酸化导致钙的结合能力提高，从而利于钙依赖性蛋白酶对蛋白质的降解。这提示可以在缺乏PEST蛋白裂解系统的E.coli中表达PEST富含蛋白。 减少E.coli中重组蛋白裂解的策略有以下几种：(1)将蛋白质靶向细胞周质或培养基[145，186];(2)在较低的温度下培养细菌[187
哺乳动物细胞和悬浮哺乳动物细胞的培养 ◆疫苗生产中病毒颗粒的制备 ◆支持杂交瘤形成时的细胞融合 ◆支持单核细胞、巨噬细胞、上皮细胞和成纤维细胞系的细胞生长 适应的细胞类型 Cell lines and Cell types ● 235-1,MNQ Rat Pituitary 大鼠垂体脑细胞 ● B82 Mouse L cells –connective tissue 小鼠L细胞联结组织 ● COS1, COS
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