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- xy-A084Hu01
- 2025年07月16日
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- 技术资料
- 保存条件：
-20℃
- 英文名：
Recombinant Leptin (LEP)
- 库存：
1000
- 供应商：
上海信裕
瘦素(LEP)重组蛋白
Recombinant Leptin (LEP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy-A084Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 46.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Val22~Cys167 with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
瘦素(LEP)重组蛋白 SEQUENCES
The sequence of the target protein is listed below.
VPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
VPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
USAGE
Reconstitute in sterile ddH2O.
瘦素(LEP)重组蛋白 STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
瘦素(LEP)重组蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
人瘦素（LEP ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 瘦素（LEP ） 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人瘦素（ LEP ）水平。用纯化的人瘦素（ LEP ）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 瘦素 （ LEP ），再与 HRP 标记的瘦素（ LEP ）抗体结合，形成
；同一病毒株可在多种宿主细胞上生长，一种细胞系平台也可能生产多种病毒；同一细胞不同培养方式病毒量、抗原结构、免疫源性、毒力等也可能不同。如狂犬病毒PM毒株从兔脊髓生产，发展到脑组织，人二倍体细胞株、MRC-5细胞、MDCK适应到现在的Vero细胞，使得狂犬疫苗的生产率明显提高；而狂犬病毒Flury LEP株从最初的鸡胚细胞生产发展到BHK21细胞生产并进一步适应了反应器悬浮培养等。 当病毒对一些细胞基质不够敏感时，有可以通过传代驯化的方式，使病毒逐步适应该细胞基质，最终在一定代次后达到一定的滴度
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瘦素(LEP)重组蛋白
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