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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
+20°C
- 保质期:
1年
- 英文名:
Fluorescein (free acid)
- 库存:
大量
- 供应商:
欣博盛
- 规格:
1 kg
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文献和实验Fluorescein labeling of proteins
from free Fluorescein compounds by extensive dialysis versus PBS or by gel filtration.Concentrate with Centriprep and/or Centricon concentrators as necessary. Microfuge on high for 10 minutes and filter through 0.22 µm filter.Optionally add 0.5% azide
Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay
The efficiency of incorporation of fluorescein-11-dUTP with both random prime labeling of long probes (Chapter 8 ) and 3′-tailing of oligonucleotide probes (Chapter 11 ) can be estimated using the following rapid labeling assay. The basic
Fluorescein Diacetate (FDA ) 1. Place pollen on slide. 2. Incubate in humid chamber (wet Whatman in Tupperware) ~ 30 min. 3. Add drops of FDA (2mg/ml in acetone) to Pollen Growth Media (~/ml
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