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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX628898
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IP
- 浓度:
0.98 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RFP
- 抗体英文名:
RFP antibody [GT1433]
- 抗体名:
RFP 抗体 [GT1433]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (GTX628898) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628898) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.
RFP antibody detects RFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
RFP antibody (GTX628898) dilution: 1:10000
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文献和实验Klymenko Y et al., Neoplasia 2017 (PMID:28601643)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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