- ￥6100
- GeneTex
- 美国
- GTX60347
- 2025年07月11日
- WB, ICC/IF, ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminal part of the protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60347
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA, ChIP assay
- 浓度：
4 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A
- 抗体英文名：
Histone H2A antibody - ChIP grade
- 抗体名：
Histone H2A 抗体 - ChIP grade
- 规格：
50 μg
ELISA analysis of peptides used for immunization using GTX60347 Histone H2A antibody - ChIP grade.
ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60347 Histone H2A antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and EIF4A2 promoters, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60347 Histone H2A antibody - ChIP grade.
Dilution : 1:2,000
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60347 Histone H2A antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500
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文献和实验Chromatin Immunoprecipitation (ChIP)
of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde
. However, to address in detail what happens at specific sites in vivo, chromatin immunoprecipitation (ChIP) is the method of choice. Here, we describe how ChIP can be performed on non-fixed chromatin from animal cells or tissues (fresh or frozen) to analyze histone
MPG (3µl/ml of media described in (Morris et al. 2004)(note 3). .Pulldown (1), Antibody specific Forty-eight hours following the biotin linked sense or antisense siRNA transfection the cultures are collected and a chromatin immunoprecipitation (ChIP
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