- ￥5300
- GeneTex
- 美国
- GTX54527
- 2025年07月15日
- WB, ICC/IF, IHC-P, FACS
- Rabbit
- Human
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- 详细信息
- 技术资料
- 免疫原：
Purified partial recombinant GATA3 protein expressed in E. coli
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX54527
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
GATA3
- 抗体英文名：
GATA3 antibody
- 抗体名：
GATA3 抗体
- 规格：
100 μg
ICC/IF analysis of MCF7 (left panel) and SH-SY5Y cells (right panel) using GTX54527 GATA3 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX54527 GATA3 antibody.
Dilution : 1:1000
ICC/IF analysis of MCF-7 cells using GTX54527 GATA3 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 1 μg/ml in 0.1% BSA incubated for 3 hours at room temperature
WB analysis of whole cell extracts (20 ug lysate) of SH-SY5Y (Lane 1), Jurkat (Lane 2) and K-562 (Lane 3) using GTX54527 GATA3 antibody.
Dilution : 1:500-1:1500
WB analysis of 20 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX54527 GATA3 antibody.
Dilution : 1:1000
IHC-P analysis of human tonsil tissue using GTX54527 GATA3 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200
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