- ￥5300
- GeneTex
- 美国
- GTX54517
- 2025年07月13日
- WB, ICC/IF, FACS
- Rabbit
- Human, Mouse, Rat, Chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Chicken
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54517
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Vinculin (phospho Tyr100)
- 抗体英文名：
Vinculin (phospho Tyr100) antibody
- 抗体名：
Vinculin (phospho Tyr100) 抗体
- 规格：
100 μl
WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).
WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1) and HeLa treated with 100ng/ml of LPS for 20mins (Lane 2) using GTX54517 Vinculin (phospho Tyr100) antibody.
Dilution : 1:1000
ICC/IF analysis of HeLa cells using GTX54517 Vinculin (phospho Tyr100) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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文献和实验to about 100μl (for 2 mg vinculin)using a Centricon-30 (concentrate in a SS34 rotor at 6,500 rpm,4℃ for about 20 min.Then collect at 2,000 rpm for 3 min). 10.Dialyze overnight against buffer 6 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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