- ￥5300
- GeneTex
- 美国
- GTX55412
- 2025年07月16日
- WB
- Rabbit
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from human E2F1.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX55412
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
E2F1 (phospho Thr433)
- 抗体英文名：
E2F1 (phospho Thr433) antibody
- 抗体名：
E2F1 (phospho Thr433) 抗体
- 规格：
100 μl
WB analysis of extracts from mouse brain tissue using GTX55412 E2F1 (phospho Thr433) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
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