- ￥1700 - 4000
- GeneTex
- 美国
- GTX131263
- 2025年07月10日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX131263
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Specific for GAC but not KGA isoform
- 抗体英文名：
Glutaminase C (GAC) antibody
- 抗体名：
Glutaminase C (GAC) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody (GTX131263) or Glutaminase C antibody (GTX132402).
Western blot analysis was performed using Glutaminase C antibody (GTX131263).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutaminase C antibody detects Glutaminase C protein at cytoplasm on mouse duidenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duidenum.
Glutaminase C antibody (GTX131263) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody (GTX131263) diluted at 1:500.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献和实验Saladini S et al., Cells 2019 (PMID:30646605)
Generation of Antibody Molecules Through Antibody Engineering
Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
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