- ￥1700 - 4000
- GeneTex
- 美国
- GTX108058
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX108058
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.66 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
VPS35
- 抗体英文名：
VPS35 antibody [C3], C-term
- 抗体名：
VPS35 抗体 [C3], C-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: VPS35 stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
VPS35 antibody detects VPS35 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
VPS35 antibody (GTX108058) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108058 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Lenoir M et al., Nat Commun 2018 (PMID:29520003)
Succoio M et al., J Proteomics 2015 (PMID:26206181)
Miura E et al., Neurobiol Dis 2014 (PMID:25107340)
Rahman AA et al., Neuroscience 2020 (PMID:32540366)
Beilina A et al., Cell Rep 2020 (PMID:32375042)
补体成分 C3(C3a/C3b) 补体成分：C3 血清浓度(μg/ml)：550-1200 分子量(kDa)：195 亚单位(链)及分子量(kDa)：α∶110；β∶85 激活产物：C3a；C3b 生物学活性：C3a 过敏毒素、趋化作用等。 C3b 组成CP、AP中的C3、C5转化
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠补体 C3(C3)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 C3 单抗包被于酶标板上，标准品和样品中的 C3 与单抗结合，加入生物素化的抗大鼠 C3 ，形成免疫复合物连接在板上，辣根
人补体C3 (C3)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C3 单抗包被于酶标板上，标准品和样品中的 C3与单抗结合，加入生物素化的抗人 C3 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测
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