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- 详细信息
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3)
- 库存：
1000
- 供应商：
上海信裕
发育全能关联蛋白3(DPPA3)重组蛋白
Recombinant Developmental Pluripotency Associated Protein 3 (DPPA3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 21.6kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Met1~Pro159 with two N-terminal Tags, His-tag and T7-tag |
发育全能关联蛋白3(DPPA3)重组蛋白
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The sequence of the target protein is listed below.
MDPSQFNPTY IPGSPQMLTE ENSRDDSGAS QISSETLIKN LSNLTINASS ESVSPLSEAL LRRESVGAAV LREIEDEWLY SRRGVRTLLS VQREKMARLR YMLLGGVRTH ERRPTNKEPK GVKKESRPFK CPCSFCVSNG WDPSENARIG NQDTKPLQP
MDPSQFNPTY IPGSPQMLTE ENSRDDSGAS QISSETLIKN LSNLTINASS ESVSPLSEAL LRRESVGAAV LREIEDEWLY SRRGVRTLLS VQREKMARLR YMLLGGVRTH ERRPTNKEPK GVKKESRPFK CPCSFCVSNG WDPSENARIG NQDTKPLQP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
发育全能关联蛋白3(DPPA3)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
发育全能关联蛋白3(DPPA3)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
4.29 丁香实验科研午间快讯（每日更新）①中国科学家 4 月参与发表多篇 Nature 及其子刊文章4 月中国学者参与的多项研究在Nature杂志及其重要子刊上发表，其中包括植物类受体蛋白激酶 TMK1 介导的生长素信号途径，测定早期肝细胞癌的蛋白质组表达谱和磷酸化蛋白质组图谱，以及首次使用RNA模板实现同源重组修复。生长素作为植物最重要的激素之一调控了复杂的植物发育过程。不同浓度的生长素对植物的调控完全不同，但其浓度效应的作用机制目前尚不清楚。来自福建农林大学海峡联合研究院园艺中心，中科
屋尘变应原。浸棒条检测更具方便、简捷的特征，10min就有检测结果。此外，还有胶体金试验等。这些技术的发展均有利于环境的检测工作，而检测工作又促使设计出更好的避免接触变应原的装置。 2．重组变应原与免疫治疗 (1)变应原重新设计与选择：cDNA克隆重组变应原及氨基酸序列的研究对于发展新型变应原特异性治疗有促进作用。显然，多价重组变应原完全能代替传统免疫治疗所用的变应原提取物，通过对螨类、猫或花粉等变应原的修饰及临床使用，已取得了令人满意的效果。重组变应
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发育全能关联蛋白3(DPPA3)重组蛋白
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