- 询价
- PEPROTECH
- 中国/美国
- xy-B596Hu01
- 2025年11月21日
13 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Phospholipase C Beta 1, Phosphoinositide Specific (PLCb1)
- 库存：
1000
- 供应商：
上海信裕
磷脂酰肌醇特异性磷酯酶Cβ1(PLCβ1)重组蛋白
Recombinant Phospholipase C Beta 1, Phosphoinositide Specific (PLCb1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 21.8kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu316~Gly476 with two N-terminal Tags, His-tag and T7-tag |
磷脂酰肌醇特异性磷酯酶Cβ1(PLCβ1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
EDMSQ PLSHYFINSS HNTYLTAGQL AGNSSVEMYR QVLLSGCRCV ELDCWKGRTA EEEPVITHGF TMTTEISFKE VIEAIAECAF KTSPFPILLS FENHVDSPKQ QAKMAEYCRL IFGDALLMEP LEKYPLESGV PLPSPMDLMY KILVKNKKKS HKSSEG
EDMSQ PLSHYFINSS HNTYLTAGQL AGNSSVEMYR QVLLSGCRCV ELDCWKGRTA EEEPVITHGF TMTTEISFKE VIEAIAECAF KTSPFPILLS FENHVDSPKQ QAKMAEYCRL IFGDALLMEP LEKYPLESGV PLPSPMDLMY KILVKNKKKS HKSSEG
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
磷脂酰肌醇特异性磷酯酶Cβ1(PLCβ1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
磷脂酰肌醇特异性磷酯酶Cβ1(PLCβ1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
完全活化成为效应T细胞。在正常生理情况下，共刺激分子与共抑制分子之间的平衡，使T细胞的免疫效应保持适当的深度、广度，从而最大程度减少对于周围正常组织的损伤，维持对自身组织的耐受、避免自身免疫反应。然而，肿瘤细胞可异常上调共抑制分子PD-1、PD-L1的含量。PD-L1与PD-1结合后，PD-1胞质区ITSM结构域中的酪氨酸发生磷酸化，募集SHP-2磷酸酶，使下游的Syk和磷脂酰肌醇3激酶(PI3K)发生去磷酸化，从而传递抑制性信号抑制淋巴细胞的功能及细胞因子的释放，打破共刺激分子与共抑制分子之间
技术资料暂无技术资料 索取技术资料
磷脂酰肌醇特异性磷酯酶Cβ1(PLCβ1)重组蛋白
询价
