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- 2026年06月04日
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- 文献和实验
- 技术资料
- 英文名：
Maltose binding protein (aa 10-375),MW = 40.3 kDa
- 供应商：
鼎国
- 库存：
大量
- 规格：
1mg
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文献和实验Purification of MBP (maLTose-binding proteins) Fused Proteins
with 10 fractions of 10 mM maltose in 20 mM Tris pH 7.4, 200 mM NaCl, 10 mM b ME, 1 mM EDTA or in some other buffer of choice, depending on subsequent purification steps. If the fusion protein is to be cleaved with factor Xa as the next step
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
TRICINE GELS Protocol (For Low MW proteins (
are very active in SDS sample buffer and can cause severe degradation. Once heated, sample could sit at RT for a short time until loading, or at -20°C for a long time. For a gel thickness of 0.75mm and 15 wells applied 10-25ug protein of a complex mixture
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