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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Synaptosomal Associated Protein 25kDa (SNAP25)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 27.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Met1~Gly206 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验100-18B Human FGF-basic,10mM Tri(pH 8.0)用于溶解100-25 Human FGF-8。 关于PeproTech公司重组细胞因子和蛋白溶解的具体方法和注意事项请见:http://www.biomart.cn/experiment/430/488/490/492/31321.htm PeproTech公司简介: PeproTech Inc. 创立于1988年,始终致力于为生命科学研究开发重组蛋白及相关产品
blotting检测,尽管如此,含有血清的组织裂解物和组织培养上清中含有免疫球蛋白,还原变性样本中含IgG,在western blot检测中则结合出现IgG分子50 and 25 kDa的重链和轻链条带。 4. 内参选择 三、抗体的保存 1. 抗体的稳定性 (1)抗体的稳定性是自然界长期进化的结果。抗体是免疫系统中最重要的分子之一,其稳定性是自然进化筛选的结果。在众多物种中,抗体分子形成了保守而稳定的结构。 (2)抗体分子的高Tm值。在室温
322的高拷贝质粒似乎并没有好处。而且已有资料表明,增加质粒的拷贝数降低E.coli中胰酶的产量,在高拷贝质粒中,强启动子的存在严重影响了细胞的活性[25,26]。 转录水平调控 1.启动子 能在E.coli中发挥作用的启动子很多。这些启动子必须具有适合高水平蛋白质合成的某些特性。 首先启动子的作用要强,待表达基因的产物要占或超过菌体总蛋白的的10-30%; 第二,它必须表现最低水平的基础转录活性。若要求大量的基因表达,最好选用高密度培养细胞和表现最低活性的可诱导和非
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