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- 2025年12月08日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Sp100 Nuclear Antigen (Sp100)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 27.6kDa |
| Concentration | 5.8 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Phe116~Pro333 (Accession # Q5XI80) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FERVL PNGLCFQRSD GGDPNSQLSL EQGPNGSCSQ ATLTLSPFNH SFSDGWGSND RLNSSLTHGN QSENHQLPGS PNHLGAVQLN HDLQINPCSV RLVDIKKENI SYSLDDNQQT QARTNENQGS EIIELSSDED SNDEINLSEA STLILSKPET AHSRQPPTPR TYRRGDTSDT SSSSLDRRRK RTGRMLSSSS SELSSGEESE EFLRSGSGRL GCP
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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- 作者
- 内容
- 询问日期
文献和实验Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.
细胞检测系统：在细胞悬浮液中加入硫化氨基酸，由于占位不同，结合在幼稚细胞的氨基酸比成熟细胞多。且对溶血剂有抵抗作用，当加入溶血剂时。成熟细胞被溶解，只保留着可能存在的幼稚细胞用来计数。Sysmex公司的SE一9000血细胞分析仪就是采用这种方法进行五分类的。该仪器可显示及打印四个直方图、两个散点图。一个报告表以及FLAG信息等。但该仪器配套的Labman中文软件却无法打印散点图。SE一9000血细胞分析仪有监测造血干细胞的功能。SE一9000血细胞分析仪和该公司研制的SP100自动制片染色机以及R3000／
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