- ¥8.84
- Pharmacia原装
- DH291-1
- Pharmacia原装
- 2025年07月16日
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- 供应商：
鼎国昌盛
- 规格：
750ml
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting
Kamps's Western Blotting Protocol
from the shelf (Margie's).Stain for approximately 20 to 60 minutes.Rinse with water for 1 hr.Dry flat.Be careful.Buffers containing Tween 20 can reduce the binding of antiphosphotyrosine antibodies. Recipes Transfer buffer: Glycine 57.6 g Tris Base 12.0 g
C. elegans whole mount immunohistochemistry with Bouin's Fixative
C. elegans whole mount immunohistochemistry with Bouin's Fixative I have developed a fixation protocol which provides an alternative set of conditions to test antisera that work poorly on paraformaldehyde
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