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文献和实验Phosphoamino acid analysis:Mark Kamps's method
or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting
C. elegans whole mount immunohistochemistry with Bouin's Fixative
in my lab). The supernatant, save 50 µl, is removed by aspiration. A mix of 400 µl of Bouin's, 400 µl methanol and 10 µl of ßME is placed in the glass tube to initiate fixation and subsequently the worms are transferred to an eppendorf tube using a glass
Collector,Pharmacia) (4)层析记录仪(Chart Recorder, Pharmacia) (5)层析柱(Hiprep 26/60 Sephacryl S-400 Size exclusion column, Pharmacia) 2、 试剂 层析缓冲液:20mM Tris(PH8.0);150mM NaCl (4 o C预冷) 【操作步骤】 (1)准备好足够量的层析缓冲液,按照操作程序接好层析装置,将进样口与出样
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