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文献和实验大,酸度愈小。 pH=7 ,溶液呈中性。当溶液的[H ]>1mol/L时,一般用[H ],而不用pH值表示溶液的酸碱性。溶液的pH值,通常用酸碱指示剂、pH试纸测定,最精确的测定方法是用pH计。溶液的酸碱性也可以用溶液中[OH - ]的负对数来表示,即pOH=-lg[OH - ]。在25℃,水的离子积是Kw=1×10 -14 ,由此可推知pH pOH=14。由pH值可推算pOH值。pOH<7是碱性溶液,pOH=7是中性溶液,pOH>7是酸性溶液。
Immobilized pH gradient (IPG) as first dimension
strips were prepared by Pharmacia LKB Biotechnology AB and are commercially available. The strips were 3 mm wide and 180 mm long. In addition, the geometry of the immobilized pH gradient strips has been changed to allow the use of large sample application
Advantages of Immobilized pH Gradients
Dried gel strips containing immobilized pH gradients (IPG) were commercially introduced in 1991 (Pharmacia Biotech, Immobiline� DryStrip gels). Their adoption for the first dimension of 2-D electrophoresis has produced significant
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