Guanidine Thiocyanate 异硫氰酸胍

RNA Extraction by the Guanidine Thiocyanate Procedure

This method relies on the strong chaotropic nature of the reagents involved to completely denature any ribonuclease (RNase) present in the sample. After lysis in guanidine thiocyanate buffers there are two possibilities for isolation

Acid-guanidinium thiocyanate-phenol-chloroform RNA purification

    N.B: Care must be taken when handling solutions containing high concentrations of guanidine salts due to its chaotropic nature. As with other procedures involving RNA, gloves should be worn at all times to avoid contamination of samples

Molecular and Biochemical Assays of Cartilage Components

agitation at 4�C. Shorter agitation times can give inadequate solubilization. The guanidine HCl-insoluble pellet must be re-extracted with guanidine thiocyanate buffer, to solubilize RNA additionally. The final insoluble pellet can be rinsed with ethanol