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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
unless otherwise specified. bFGF solution (10 µg/mL) Collagenase solution (1 mg/mL) Freezing medium for primary cells Gelatin solution (0.1%, w/v) for MEF hESCs (cultured in a six-well plate) Knockout DMEM (Invitrogen 10829-018
or anhydrous salts c S. Ringer, J. Physiol. (London) 18 , 425 (1895) d M.V. Tyrode, Arch. Int. Pharmacodyn. Ther., 20 , 2025 (1910
PriCells: Methods of Isolation and Culture of Human Hepatocytes
perfusion modified from the method, which was derived from the method. The major modifications were the substitution of EBSS for HEPES as the perfusion buffer and an additional washing step to remove residual EGTA prior to collagenase perfusion, adopted
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