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文献和实验Fluorescent In Situ Transcription in Cells and Tissues
transcript using reverse transcriptase and labeled through incorporation of a fluorescent?labeled dUTP nucleotide base. The new cDNA will be synthesized upstream from the point of primer hybridization, and has a specific activity of fluorescent labeling
Fluorescence-Based Technique for Analyzing Retinoic Acid
retinoic acid-binding protein I (CRABP-I), a protein that binds RA with high affinity and specificity, as a “read-out” for its ligand. Replacing 28 Leu of CRABP-I with a Cys residue allows for covalently attaching an environmentally sensitive
Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein
Green fluorescent protein (GFP) of the jellyfish Aqueorea victoria is a 238-amino-acid, 28-kDa protein that absorbs light with an excitation maximum of 395 nm and fluoresces with an emission maximum of 509 nm (1 ). GFP owes its unique
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