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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
20 cm cell, inner & outer plates
- 规格:
2
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
. Procedure Prepare petri plates with 0.4 cm of base agar medium. LB (or other nutrient) with 1.2% agar for bacteria YPD (or other nutrient) with 2% agar for yeast Prepare 0.7% agar medium (soft agar
of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap
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