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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Recombinant Mouse BH3 Interacting Domain Death Agonist GST
- 库存：
大量
- 供应商：
北京鼎国昌盛
- 规格：
2µg
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文献和实验Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
Affinity Purification of Proteins Binding to GST Fusion Proteins
Figure 20.2.2 Purification of an interacting test protein from a crude E. coli extract (−80)/(+80). Crude E. coli extracts from control cells (−80) or cell sexpressing the yeast negative regulator Gal80p (+80) were incubated with a GST fusion
with recombinant GST fusion proteins should be replicated with GST alone. A bait protein with a mutation in the predicted interaction domain can be processed as a control to determine specificity of the protein:protein interaction. A non-relevant protein
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