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文献和实验A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
a background and then counted as specific spots. We have tested a cell detachment reagent, Accumax™, and found that it may be used to remove a large number of cells adhered to the microplate membranes. Accumax was tested in 16 different ELISPOT assays
normally cease when confluence is reached. Harvesting cells for study, processing or subculture requires dissociation and detachment of the monolayer. Limited treatment of the cell layer with the enzyme trypsin is the method most frequently applied
This protocol describes the culture of human pluripotent stem cells (PSCs) under feeder-free conditions in a commercially available, chemically defined, growth medium, using Matrigel as a substrate and the enzyme solution Accutase for single
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