PE BrdU Staining Kit for Flow

【共享】流式 Overview of Flow Cytometry

. First is the pre–flow cytometry phase which involves reagent preparation, cell preparation, protocol design, and staining of cells with the fluorescent reagents. Second is the flow cytometry phase which involves processing the stained cells using flow cytometry

A general protocol for staining cell for cytometry analysis

2 µl PI and incubate for 15 min at RT. 5.Add 400 µl of 1× Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr). Note: Methods for utilizing Annexin V binding on adherent cells ( i.e., monolayer

Intracellular Immunofluorescent Staining for Flow Cytometry

. No. 00-8222 ) eBioscience Permeabilization Buffer (Cat. No. 00-8333 ) eBioscience Flow Cytometry Staining Buffer (Cat. No. 00-4222 ) Instruments Pipettes and pipettors Centrifuge Ice bucket or refrigerator Flow