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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
BrdU Staining Buffer Set for Flow Cytometry Kit
- 规格：
KIT
BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis.
This set in combination with BrdU and DNase I steps allows for detection of BrdU
pulse-labeled cells thereby enabling the examination of proliferating cells.
Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic
analog of thymidine which incorporates into newly synthesized genomic DNA during
the S-phase of mitosis. Following DNA denaturation, the cells are stained for
BrdU incorporation along with any other cell surface and/or intracellular targets of interest.
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文献和实验A general protocol for staining cell for cytometry analysis
2 µl PI and incubate for 15 min at RT. 5.Add 400 µl of 1× Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr). Note: Methods for utilizing Annexin V binding on adherent cells ( i.e., monolayer
Intracellular Immunofluorescent Staining for Flow Cytometry
. No. 00-8222 ) eBioscience Permeabilization Buffer (Cat. No. 00-8333 ) eBioscience Flow Cytometry Staining Buffer (Cat. No. 00-4222 ) Instruments Pipettes and pipettors Centrifuge Ice bucket or refrigerator Flow
Supplies: Ethanol 100 USP (highest quality) FACS Staining Buffer (1XPBS w/ 3 calf serum, 0.05 azide--filtered) ―Dilute staining antibodies in Buffer DNAse (Sigma D-5025, Bovine Pancreas) RNase (Boehringer, 25 mg bovine pancrease)
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