手机验证
大量供应
康朗生物
详见说明书
夹心法
ELISA 定量检测
详见说明书
血清,血浆,尿液 细胞裂解液,组织匀浆,细胞培养上清液
96T/盒
Product name: | Human Elastase 3A (ELA3A) ELISA Kit |
Method: | sandwich |
Synonyms: | MYF4; Myogenic Factor 4 |
Catalog number: | DL-ELA3A-Hu |
Detection range: | 0.312-20ng/mL |
Size: | 96T |
Assay length | 1-4.5Hours |
Price: | inquiry |
Quality guarantee period: | for 12 months |
Item | Standard | Test Result | |
Description | This immunoassay kit allows for the specific measurement of this index in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and Other biological fluids.. |
Conform | |
Identification | Colorimetric | Positive | |
Composition | Pre-coated, ready to use 96-well strip plate Standard (freeze dried) Standard Diluent Detection Reagent A Detection Reagent B Assay Diluent A Assay Diluent B TMB Substhumane Stop Solution Wash Buffer(30 x concenthumane) Plate sealer for 96 wells Instruction manual |
1 2 1 × 20ml 1× 120μl 1× 120μl 1 × 12ml 1 × 12ml 1 × 9ml 1 ×6ml 1 ×20ml 2 1 |
Conform |
The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve
Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-93 | 86 |
EDTA plasma(n=5) | 80-97 | 88 |
heparin plasma(n=5) | 90-101 | 95 |
The linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37℃;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37℃;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
DL-MGP-Hu 人基质Gla蛋白(MGP) ELISA Kit human Matrix Gla Protein (MGP) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.7pg/mL 96T 3705 12 months
DL-HBEGF-Hu 人肝素结合性表皮生长因子(HBEGF) ELISA Kit human Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.3pg/mL 96T 3180 12 months
DL-GHRL-Hu 人饥饿素(GHRL) ELISA Kit human Ghrelin (GHRL) ELISA Kit Homo sapiens human 123.46-10000pg/mL 47.2pg/mL 96T 3530 12 months
DL-GHRL-Ra 大鼠饥饿素(GHRL) ELISA Kit Rat Ghrelin (GHRL) ELISA Kit Rattus norvegicus Rat 123.46-10000pg/mL 42.6pg/mL 96T 3800 12 months
DL-GHRL-Mu 小鼠饥饿素(GHRL) ELISA Kit mouse Ghrelin (GHRL) ELISA Kit Mus musculus mouse 123.46-10000pg/mL 42.5pg/mL 96T 3620 12 months
DL-dSIP-Hu 人δ-促睡眠肽(δSIP) ELISA Kit human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit Homo sapiens human 98.77-8000pg/mL 37.58pg/mL 96T 3530 12 months
DL-ALDOS-Ra 大鼠醛固酮合酶(ALDOS) ELISA Kit Rat Aldosterone Synthase (ALDOS) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.063ng/mL 96T 3800 12 months
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。