- 询价
- ZYMO RESEARCH
- D1035
- 2026年01月02日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
5-Methyl Dctp [10mM]
- 供应商：
简石生物
HIGHLIGHTS
- Ready-to-use, ultra pure 5-Methylcytosine dCTP; >99% trisphosphate as determined by HPLC analysis.
- Readily incorporates into PCR amplicons with ZymoTaq or other DNA polymerases.
- Free of endo/exo-deoxyribonucleases, ribonucleases, and phosphatases.
DESCRIPTION
5-Methyl dCTP can be used in nucleotide mixtures to generate PCR amplicons using ZymoTaq, QuestTaq, or other DNA polymerases.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验You will need for 1° cDNA synthesis: 5 x SuperScript RTase buffer (Gibco-BRL) 5mM methyl dNTPs (Pharmacia, substitute 5m dCTP for dCTP) 100mM DTT (Gibco-BRL) oligo dT primer/adaptor [a-32P] dATP (~400Ci/mmol, Amersham or DuPont) Ribonuclease inhibitor
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
PCR Protocol 10X PCR Buffer 1.0MgCl2 (2.0mM) 0.8µldNTPS mix* 0.8µlPrimer-F (µg/µl) 0.1µlPrimer-R (µg/µl) 0.1µlTaq (5U) 0.1µl32P dCTP (10µci/µl) 0.1µlddH2O 4.5µlDMSO (100%) 0.5µlSUBTOTAL 8.0µlTemplate DNA (50-100ng) 2.0µlNote: Synthesize primers 21
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
dTTP 5 mM 100mM 50 µL dATP 25mM 100mM 250 µL dGTP 25mM 100mM 250 µL dCTP 25mM
