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- 文献和实验
- 技术资料
- 库存:
1000
- 供应商:
康朗生物
- 检测范围:
见说明书
- 检测方法:
酶联免疫
- 应用:
科研
- 标记物:
HRP标记物
- 样本:
血清/组织/尿液
Human angiotensinogen (aGT) ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.533 2.589 2.561 2.467
2500 2.234 2.268 2.251 2.157
1250 1.618 1.628 1.623 1.529
625 1.005 1.014 1.010 0.916
312 0.423 0.453 0.438 0.344
156 0.236 0.243 0.240 0.146
78 0.149 0.156 0.153 0.059
0 0.091 0.096 0.094
Human angiotensinogen (aGT) ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human angiotensinogen (aGT) ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:200 Average % 90
Range % 85-100
1:400 Average % 97
Range % 91-105
1:800 Average % 98
Range % 93-110
1:1600 Average % 93
Range % 85-105
Human angiotensinogen (aGT) ELISA Kit
回收率:The recovery of human aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-100
EDTA plasma (n=4) 97 90-105
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文献和实验调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
提供多种高品质补体相关试剂,助力广大科研工作者的深入探索与研究。 以下是菲恩生物 补体 相关指标产品推荐 ELISA 试剂盒 货号 产品名称 灵敏度 EH5309 Human Anti-C3b antibody ELISA Kit 0.938ng/ml EH5308 Human Anti-CFB antibody ELISA Kit 0.469ng/ml EH5255
推荐: ELISA 试剂盒 货号 产品名称 灵敏度 检测范围 EH0184 Human IL-1α ELISA Kit 2.344pg/ml 3.906-250pg/ml (1pg=160mIU) QT-EH0184 Human IL-1α QuickTest ELISA Kit 2.344pg/ml 3.906-250pg/ml (1pg=160mIU) QT-EH0185-1S
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