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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
干燥、避光、密闭、低温(0-10℃
- 英文名:
1-O-Deacetyl-2alpha-hydroxykhayanolideE
- 库存:
10
- 供应商:
上海古朵生物
- 规格:
mg/g
货号:GD-YW1533
CAS:1189801-51-1
通用名:1189801-51-1,1-O-Deacetyl-2alpha-hydroxykhayanolideE
英文名称:1-O-Deacetyl-2alpha-hydroxykhayanolideE
中文名称:
包装:提供多种包装规格并可按要求定制。
纯度:98%以上;
储存:干燥、避光;
库存:现货(库存数量有时会因销量问题有所波动,具体请联系在线销售!)
用途:本品仅用于实验室科研含量测定。
古朵生物现货供应,1-O-Deacetyl-2alpha-hydroxykhayanolideE1189801-51-1标准品价格优惠、质量可靠、产品试验效果好得到了科研单位的好评,1-O-Deacetyl-2alpha-hydroxykhayanolideE1189801-51-1标准品
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文献和实验Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
streptomycin, 37o C. 3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech, 08-110). Stored as 250 ul aliquots in 15 ml conical tubes at -80o C. Keep it cold all the time otherwise it will form a gel. 4. F12K medium (no additives), for diluting
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