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文献和实验pmol分子量100,000蛋白质=10μg 100pmol分子量50,000蛋白质=5μg 100pmol分子量10,000蛋白质=1μg 氨基酸的平均分子量=126.7道尔顿 (5)蛋白质/DNA换算: 1kb DNA=333 个氨基酸编码容量=3.7×104MW蛋白质 10,000MW蛋白质=270bp DNA 30,000MW蛋白质=810bp DNA 50,000MW蛋白质=1.35kb 100,000MW蛋白质=2.7kb DNA4.常用蛋白质分子量标准参照
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1523 实验步骤 1. Construction of a laser ablation system Wear laser safety goggles and use good laser safety practices during the initial alignment. Never look through the oculars
of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application. The kit is also available in spin-column format for micro- and mini-sized samples. Principle and procedure
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