缓冲液/成分:1X PBS pH 7.2;1X protease inhibitor cocktail;1 mM PMSF
Volume: 50μl/ 250μl/ 500μl
体积:50μl/ 250μl/ 500μl
Concentration: 2 μg/μl
浓度:2 μg/μl
Storage: Short Term: 4℃ Long Term (up to 1 year): -20℃(avoid repeated freeze/thaw)
储存:短期:4℃长期(可达1年):-20℃(避免反复冻融)
Coomassie stained gel showing purification of recombinant NS3 helicase protein.Bacterial cells expressing the His-tagged protein were lysed (lane 2), pelleted, and the SN (lane 3) was loaded onto Nickel resin. The ~55 kD protein was absent in the resin flow thru (lane 4) as well as column washes (lanes 5 and 6). Elutions with immidazole (lanes 7 and 8) yielded ≥95% pure protein.Lane 1 = marker