Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min