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    研选PARP antibody [N2C1], Internal

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    PARP antibody [N2C1], Internal

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    • 商家诚信度 
    • 入驻年限  7年
    • 2456 Alton PkwyIrvine, CA 92606 USA
      • 货号:

        gtx112864

      • 应用范围:

        WB,ICC/IF,IHC-P,IP,ChIP assay

      • 适应物种:

        Human,Mouse,Rat

      • 宿主:

        Rabbit

      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: PARP protein stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
      Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
      Blue: Hoechst 33342 staining.
      Scale bar = 10 μm.

      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      *The competitor is not affiliated with GeneTex and does not endorse this product.

      ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
      IP samples: HCT-116 whole cell extract
      A. 30 μg HCT-116 whole cell extract
      B. Control with 4 μg of preimmune Rabbit IgG
      C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
      5 % SDS-PAGE
      The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500.
      [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

      Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

      PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
      Sample: Paraffin-embedded HeLa xenograft.
      PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.

      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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