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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
Lightning-Link Biotin conjugation kit type A
- 库存:
3-4周
- 供应商:
研卉生物
- 规格:
1 reaction up to 2mg
Lightning-Link™ “一步法”抗体标记试剂盒
| 在 进行多因子检测时,我们往往因不能找到多种合适的标记一抗,而不得不选用标记二抗,因此很难避免可能发生的交叉反应及繁琐的洗涤步骤。 Lightening-LinK™抗体标记试剂盒系列,提供多种荧光蛋白、酶及荧光素标记等产品,试验操作人员可直接应用于标记一抗,从而避免了因使用二 抗带来的交叉反应及非特异性结合等问题。该系列试剂盒具有以下显著特点: |
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眼见为实! – 请点击 Lightning––Link视频 more... |
| Innova Bioscience公司Lighting-Link™系列与其他品牌的抗体标记试剂盒比较 | |||||||
| 厂 家 | Supplier I1 | Supplier I2 | Supplier I3 | Supplier S1 | Supplier S2 | Supplier D | Lighting-Link |
| 手工操作时间 | 2 分钟 | 20分钟 | 30分钟 | 30分钟 | 30分钟 | 10分钟 | 30秒钟 |
| 是否过柱 | 不需要 | 需要 | 需要 | 需要 | 需要 | 需要 | 不需要 |
| 试剂盒组分 | 6组分 | 7组分 | 5组分 | 9组分 | 12组分 | 6组分 | 3组分 |
| 操作步骤 | 5步 | 11步 | 16步 | 27步 | 31步 | 8步 | 3步 |
| 抗体标记时间 | 10分钟 | 2.5小时 | 2小时 | 1.5小时 | 4小时 | 1小时 | 2小时 |
| 可标记抗体量 | 1-20ug | 10-20ug | 20-100ug | 25ug-5mg | 100ug | 50-200ug | 100ug-5mg |
| 连接化学健 | 非共价结合* | 共价结合 | 共价结合 | 共价结合 | 共价结合 | 共价结合 | 共价结合 |
| 抗体亚型 | 仅IgG | 仅IgG | 所有 | 仅IgG | 仅IgG | 仅IgG | 所有 |
试剂盒组成:
1)1或3(以规格而定)玻管Lightening-Link™ Mix 混合物。
2)1管LL-X(X为标记物,如PE或HRP等)修饰试剂。
3)1管LL-X萃灭试剂。
操作步骤:
1)在每10ul抗体样品中加入1ul的LL-Modifier修饰试剂,轻轻混匀。
2)打开Ligtening-Link混合物管盖,吸取步骤1已混好的抗体样品至管内冻干混合物中,用吸液器轻轻吸打1-2次,使粉末完全溶解。
3)盖上玻璃瓶盖,室温(20-25°C)避光孵育3小时,或过夜。*
4)孵育3小时后,按每10ul抗体的量加入1ul LL-淬灭试剂,静置30分钟后即可使用。
*不同的标记物,操作步骤略有不同。(操作步骤供参考,以英文原版说明书为准。)
标记结合物的保存:
对于任何新结合物,推荐保持于4°C条件下。如需长期保存,可添加保护剂。
缓冲液及添加剂兼容性:
A. 与无胺基缓冲液兼容,包括MES,MOPS,HEPES(浓度10-50mM, pH范围6.5-8.5),LL-Modifier修饰剂提供结合反应的最佳条件。
B. 常见的盐(如氯化钠)、螯合剂(EDTA)、糖对偶联反应无影响。
C. 叠氮钠(0.02%-0.1%)对结合反应有轻微影响,叠氮钠不可用于HRP标记,其为后者的抑制剂。
D. 甘油(up to 50%)对结合效率无影响。
E. 若无胺基的缓冲液浓度相对较高,pH值在6.6-8.5范围外,则需要加入更多的LL-midifier修饰剂(/10ul抗体),以此来调整pH值。最佳的pH范围为7.3-7.6左右。
G. 浓度达到或稍低于20mM的Tris对结合效率有轻微的影响。
| 产品编号 | 产品名称 | 规格 |
| HRP辣根过氧化物酶抗体标记试剂盒 | ||
| 701-0010 | Lightning-Link HRP conjugation kit | 1 x 100μg HRP |
| 701-0000 | Lightning-Link HRP conjugation kit | 3 x 100μg HRP |
| 701-0002 | Lightning-Link HRP conjugation kit | 1mg HRP |
| 701-0003 | Lightning-Link HRP conjugation kit | 5 x 1mg HRP |
| 701-0004 | Lightning-Link HRP conjugation kit | 5mg HRP |
| AP碱性磷酸酶抗体标记试剂盒 | ||
| 702-0005 | Lightning-Link AP conjugation kit | 1 x 100μg AP |
| 702-0010 | Lightning-Link AP conjugation kit | 3 x 100μg AP |
| 702-0015 | Lightning-Link AP conjugation kit | 1mg AP |
| GOx葡糖氧化酶标记试剂盒 | ||
| 706-0010 | Lightning-Link GOx conjugation kit | 3 x 100μg GOx |
| 706-0015 | Lightning-Link GOx conjugation kit | 1 x 1mg GOx |
| R-PE(PE)标记试剂盒 | ||
| 703-0005 | Lightning-Link R-Phycoerythrin (R-PE) | 1 x 100μg PE |
| 703-0010 | Lightning-Link R-Phycoerythrin (R-PE) | 3 x 100μg PE |
| 703-0015 | Lightning-Link R-Phycoerythrin (R-PE) | 1mg PE |
| B-PE标记试剂盒 | ||
| 716-0005 | Lightning-Link B-Phycoerythrin (B-PE) | 1 x 100μg BPE |
| 716-0010 | Lightning-Link B-Phycoerythrin (B-PE) | 3 x 100μg BPE |
| 716-0015 | Lightning-Link B-Phycoerythrin (B-PE) | 1mg BPE |
| C-PE标记试剂盒 | ||
| 720-0005 | Lightning-Link C-Phycoerythrin (C-PE) | 1x100ug |
| 720-0010 | Lightning-Link C-Phycoerythrin (C-PE) | 3x100ug |
| 720-0015 | Lightning-Link C-Phycoerythrin (C-PE) | 1x1mg |
| APC标记试剂盒 | ||
| 705-0005 | Lightning-Link Allophycocyanin (APC) | 1 x 100μg APC |
| 705-0010 | Lightning-Link Allophycocyanin (APC) | 3 x 100μg APC |
| 705-0015 | Lightning-Link Allophycocyanin (APC) | 1mg APC |
| C-PC标记试剂盒 | ||
| 709-0005 | Lightning-Link C-Phycocyanin (C-PC) | 1 x 100ug CPC |
| 709–0010 | Lightning-Link C-Phycocyanin (C-PC) | 3 x 100ug CPC |
| 709-0015 | Lightning-Link C-Phycocyanin (C-PC) | 1 x 1mg CPC |
| R-PC标记试剂盒 | ||
| 721-0005 | Lightning-Link R-Phycocyanin (R-PC) | 1x100ug |
| 721-0010 | Lightning-Link R-Phycocyanin (R-PC) | 3x100ug |
| 721-0015 | Lightning-Link R-Phycocyanin (R-PC) | 1x1mg |
| PerCP标记试剂盒 | ||
| 718-0005 | Lightning-Link PerCP | 1x100ug |
| 718-0010 | Lightning-Link PerCP | 3x100ug |
| 718-0015 | Lightning-Link PerCP | 1x1mg |
| 荧光素标记试剂盒 | ||
| 707-0010 | Lightning-Link Fluorescein | 3 reactions (each up to 200ug) |
| 707-0015 | Lightning-Link Fluorescein | 1 reaction (up to 2mg) |
| 罗丹明标记试剂盒 | ||
| 710-0010 | Lightning-Link Rhodamine | 3 reactions (each up to 200ug) |
| 710-0015 | Lightning-Link Rhodamine | 1 reaction (up to 2mg) |
| AMCA标记试剂盒 | ||
| 712-0010 | Lightning-Link AMCA | 3 reactions (each up to 200ug) |
| 712-0015 | Lightning-Link AMCA | 1 reaction (up to 2mg) |
| Texas Red标记试剂盒 | ||
| 714-0010 | Lightning-Link Texas Red | 3 reactions (each up to 200ug) |
| 714-0015 | Lightning-LinkTexas Red | 1 reaction (up to 2mg) |
| Cy系列染标记试剂盒 | ||
| 780-0010 | Lightning-Link Cy3 | 3 reactions (each up to 200ug) |
| 780-0015 | Lightning-Link Cy3 | 1 reaction (up to 2mg) |
| 781-0010 | Lightning-Link Cy5 | 3 reactions (each up to 200ug) |
| 781-0015 | Lightning-Link Cy5 | 1 reaction (up to 2mg) |
| 782-0010 | Lightning-Link Cy5.5 | 3 reactions (each up to 200ug) |
| 782-0015 | Lightning-Link Cy5.5 | 1 reaction (up to 2mg) |
| 783-0010 | Lightning-Link Cy7 | 3 reactions (each up to 200ug) |
| 783-0015 | Lightning-Link Cy7 | 1 reaction (up to 2mg) |
| 784-0010 | Lightning-Link Cy3.5 | 3 reactions (each up to 200ug) |
| 784-0015 | Lightning-Link Cy3.5 | 1 reaction (up to 2mg) |
| Atto荧光染料标记试剂盒 | ||
| 730-0010 | Lightning-Link atto390 | 3 reactions (each up to 200ug) |
| 730-0015 | Lightning-Link atto390 | 1 reaction (up to 2mg) |
| 731-0010 | Lightning-Link atto425 | 3 reactions (each up to 200ug) |
| 731-0015 | Lightning-Link atto425 | 1 reaction (up to 2mg) |
| 732-0010 | Lightning-Link atto465 | 3 reactions (each up to 200ug) |
| 732-0015 | Lightning-Link atto465 | 1 reaction (up to 2mg) |
| 733-0010 | Lightning-Link atto488 | 3 reactions (each up to 200ug) |
| 733-0015 | Lightning-Link atto488 | 1 reaction (up to 2mg) |
| 736-0010 | Lightning-Link atto532 | 3 reactions (each up to 200ug) |
| 736-0015 | Lightning-Link atto532 | 1 reaction (up to 2mg) |
| 738-0010 | Lightning-Link atto565 | 3 reactions (each up to 200ug) |
| 738-0015 | Lightning-Link atto565 | 1 reaction (up to 2mg) |
| 739-0010 | Lightning-Link atto590 | 3 reactions (each up to 200ug) |
| 739-0015 | Lightning-Link atto590 | 1 reaction (up to 2mg) |
| 740-0010 | Lightning-Link atto594 | 3 reactions (each up to 200ug) |
| 740-0015 | Lightning-Link atto594 | 1 reaction (up to 2mg) |
| 741-0010 | Lightning-Link atto610 | 3 reactions (each up to 200ug) |
| 741-0015 | Lightning-Link atto610 | 1 reaction (up to 2mg |
| 742-0010 | Lightning-Link atto611 | 3 reactions (each up to 200ug) |
| 742-0015 | Lightning-Link atto611 | 1 reaction (up to 2mg) |
| 743-0010 | Lightning-Link atto620 | 3 reactions (each up to 200ug) |
| 743-0015 | Lightning-Link atto620 | 1 reaction (up to 2mg) |
| 744-0010 | Lightning-Link atto633 | 3 reactions (each up to 200ug) |
| 744-0015 | Lightning-Link atto633 | 1 reaction (up to 2mg) |
| 746-0010 | Lightning-Link atto637 | 3 reactions (each up to 200ug) |
| 746-0015 | Lightning-Link atto637 | 1 reaction (up to 2mg) |
| 749-0010 | Lightning-Link atto655 | 3 reactions (each up to 200ug) |
| 749-0015 | Lightning-Link atto655 | 1 reaction (up to 2mg) |
| 750-0010 | Lightning-Link atto680 | 3 reactions (each up to 200ug) | ||
| 750-0015 | Lightning-Link atto680 | 1 reaction (up to 2mg) | ||
| 751-0010 | Lightning-Link atto700 | 3 reactions (each up to 200ug) | ||
| 751-0015 | Lightning-Link atto700 | 1 reaction (up to 2mg) | ||
| FluoProbes Dyes荧光探针染料标记试剂盒 | ||||
| 788-0010 | Lightning-Link FluoProbes 547H | 3 reactions (each up to 200ug) | ||
| 788-0010 | Lightning-Link FluoProbes 547H | 1 reaction (up to 2mg) | ||
| 791-0010 | Lightning-Link FluoProbes 647H | 3 reactions (each up to 200ug) | ||
| 791-0010 | Lightning-Link FluoProbes 647H | 1 reaction (up to 2mg) | ||
| 792-0010 | Lightning-Link FluoProbes 682H | 3 reactions (each up to 200ug) | ||
| 792-0010 | Lightning-Link FluoProbes 682H | 1 reaction (up to 2mg) | ||
| 793-0010 | Lightning-Link FluoProbes 752H | 3 reactions (each up to 200ug) | ||
| 793-0010 | Lightning-Link FluoProbes7527H | 1 reaction (up to 2mg) | ||
| PE/Cy系列串联染料 | ||||
| 760-0005 | Lightning-Link PE/Cy5 | 1x100ug | ||
| 760-0010 | Lightning-Link PE/Cy5 | 3x100ug | ||
| 760-0015 | Lightning-Link PE/Cy5 | 1x1mg | ||
| 761-0005 | Lightning-Link PE/Cy5.5 | 1x100ug | ||
| 761-0010 | Lightning-Link PE/Cy5.5 | 3x100ug | ||
| 761-0015 | Lightning-Link PE/Cy5.5 | 1x1mg | ||
| 762-0005 | Lightning-Link PE/Cy7 | 1x100ug | ||
| 762-0010 | Lightning-Link PE/Cy7 | 3x100ug | ||
| 762-0015 | Lightning-Link PE/Cy7 | 1x1mg | ||
| PerCP/Cy5.5串联染料 | ||||
| 763-0005 | Lightning-Link PerCP/Cy5.5 | 1x100ug | ||
| 763-0010 | Lightning-Link PerCP/Cy5.5 | 3x100ug | ||
| 763-0015 | Lightning-Link PerCP/Cy5.5 | 1x1mg | ||
| APC/Cy系列串联染料 | ||||
| 764-0005 | Lightning-Link APC/Cy5.5 | 1x100ug | ||
| 764-0010 | Lightning-Link APC/Cy5.5 | 3x100ug | ||
| 764-0015 | Lightning-Link APC/Cy5.5 | 1x1mg | ||
| 765-0005 | Lightning-Link APC/Cy7 | 1x100ug | ||
| 765-0010 | Lightning-Link APC/Cy7 | 3x100ug | ||
| 765-0015 | Lightning-Link APC/Cy7 | 1x1mg | ||
| 生物素化结合试剂盒 | ||||
| 704–0010 | Lightning-Link Biotin conjugation kit type A | 3 reactions (up to 200μg Ab) | ||
| 704–0015 | Lightning-Link Biotin conjugation kit type A | 1 reaction (up to 2mg Ab) | ||
| 715-0010 | Lightning-Link Biotin conjugation kit type B | 3 reactions (up to 200μg Ab) | ||
| 715-0015 | Lightning-Link Biotin conjugation kit type B | 1 reaction (up to 2mg Ab) | ||
| 链亲和素标记试剂盒 | ||||
| 708-0010 | Lightning-Link Streptavidin conjugation kit | 3 x 100ug antibody | ||
| 708-0015 | Lightning-Link Streptavidin conjugation kit | 1 x 1mg antibody | ||
| 亲和素标记试剂盒 | ||||
| 717-0010 | Lightning-Link Avidin conjugation kit | 3 x 100ug Avidin | ||
| 717-0015 | Lightning-Link Avidin conjugation kit | 1 x 1mg Avidin | ||
| Trap fluorescein标记试剂盒 | ||||
| 711-0001 | Fluoro-Trap fluorescein conjugation kit | 3 x 100μg antibody | ||
| 711-0010 | Fluoro-Trap fluorescein conjugation kit | 5 x 1mg antibody | ||
| 酶结合物稳定剂 | ||||
| 901-0005 | LifeXtend HRP conjugate stabilizer | 50ml | ||
| 902-0005 | LifeXtend Alk phos conjugate stabilizer | 50ml | ||
| 马来酰亚胺活化的标记物(增强标记结合反应) | ||||
| 401-0005 | Maleimide Activated HRP | 5mgs | ||
| 402-0005 | Maleimide Activated Alkaline Phosphatase | 5mgs | ||
| 403-0005 | Maleimide Activated R-Phycoerythrin | 5mgs | ||
| 404-0005 | Maleimide Activated Allophycocyanin-XL | 5mgs | ||
| 405-0005 | Maleimide Activated Streptavidin | 5mgs | ||
| 406-0005 | Maleimide Activated Glucose Oxidase | 5mgs | ||
| 407-0005 | Maleimide Activated Ovalbumin | 5mgs | ||
| 408-0005 | Maleimide Activated BSA | 5mgs | ||
| Colorlock磷检测试剂盒 | ||||
| 302-0125 | PiColorlock ALS | 625/1560 assays | ||
| 302-0500 | PiColorlock ALS | 2500/6250 assays | ||
| 303-0030 | PiColorlock Gold | 625/1560 assays | ||
| 303-0125 | PiColorlock Gold | 2500/6250 assays | ||
| Pi结合树脂 | ||||
| 501-0015 | Pi Bind | 5g | ||
| NTP琼脂糖 | ||||
| 502-0002 | ATP agarose Low (1-2 mmol/ml) | 2ml | ||
| 502-0005 | ATP agarose Low (1-2 mmol/ml) | 5ml | ||
| 502-1000 | ATP agarose combipack | 1 pack | ||
| 503-0002 | ATP Bind | 5 x 0.4g columns | ||
| 505-0001 | GTP agarose High (>6 mmol/ml) | 2ml | ||
| 505-0002 | GTP agarose High (>6 mmol/ml) | 5ml | ||
| 506-0002 | ADP agarose Low (1-2 mmol/ml) | 2ml | ||
| 506-0005 | ADP agarose Low (1-2 mmol/ml) | 5ml | ||
| 510-0002 | ATP agarose High (8-12 mmol/ml) | 2ml | ||
| 510-0005 | ATP agarose High (8-12 mmol/ml) | 5ml | ||
| 511-0002 | ADP agarose High (8-12 mmol/ml) | 2ml | ||
| 511-0005 | ADP agarose High (8-12 mmol/ml) | 5ml | ||
| 520-0002 | Control Resin | 2ml | ||
| 520-0005 | Control Resin | 5ml | ||
| NTPase酶检测试剂盒 | ||||
| 601-0120 | ATPase assay system | 192 assays | ||
| 601-0121 | ATPase assay system | 480 assays | ||
| 601-0122 | ATPase assay system | 5 x 480 assays | ||
| 602-0120 | GTPase assay system | 192 assays | ||
| 602-0121 | GTPase assay system | 480 assays | ||
| 602-0122 | GTPase assay system | 5 x 480 assays | ||
| 其他产品 | ||||
| 850-0005 | PROTEIN A - HRP | 0.5ml (3mg/ml conjugate) | ||
| 851-0024 | Protein A – Agarose | 2ml packed vol | ||
| 854-0005 | PROTEIN A - ALKALINE PHOSPHATASE | 0.5ml (3mg/ml conjugate) | ||
| 857-0005 | STREPTAVIDIN - HRP | 0.5ml (3mg/ml conjugate) | ||
| 858-0005 | Protein A | 1mg |
| 858-0010 | Protein A | 5mg |
| 859-0005 | Goat anti-rabbit IgG - Agarose | 2ml packed vol |
| 860-0005 | AbSelect Antibody Purification Kit | 1 purification |
| 860-0010 | AbSelect Antibody Purification Kit | 3 purifications |
| 861-0005 | STREPTAVIDIN – ALK. PHOSPHATASE | 0.5ml (3mg/ml conjugate) |
| 864-0005 | STREPTAVIDIN - R-PHYCOERYTHRIN | 0.5ml (3mg/ml conjugate) |
| 865-0005 | PROTEIN A - R-PHYCOERYTHRIN | 0.5ml (3mg/ml conjugate) |
| 888-0026 | PROTEIN A - BIOTIN | 0.5ml (3mg/ml conjugate) |
| 888-0051 | STREPTAVIDIN – FLUORESCEIN | 0.5ml (3mg/ml conjugate) |
| 888-0052 | PROTEIN A – FLUORESCEIN | 0.5ml (3mg/ml conjugate) |
| 888-0077 | STREPTAVIDIN - ALLOPHYCOCYANIN | 0.5ml (3mg/ml conjugate) |
| 888-0078 | PROTEIN A - ALLOPHYCOCYANIN | 0.5ml (3mg/ml conjugate) |
| 888-0103 | STREPTAVIDIN - R-PHYCOERYTHRIN | 0.5ml (3mg/ml conjugate) |
| 888-0129 | STREPTAVIDIN– GLUCOSE OXIDASE | 0.5ml (3mg/ml conjugate) |
| 888-0130 | PROTEIN A – GLUCOSE OXIDASE | 0.5ml (3mg/ml conjugate) |
| 889-0005 | Biotinylated HRP | 0.5ml (3mg/ml conjugate) |
| 890-0005 | Biotinylated PE | 0.5ml (3mg/ml conjugate) |
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